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Recombinant Human CSF1, His-tagged

Cat.No.: CSF1-32H
Product Overview: Recombinant Human CSF1 was produced in the endosperm tissue of barley grain (Hordeum vulgare).
Description: M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological ativity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.
Source: Barley grain (Hordeum vulgare)
Species: Human
Tag: His
Form: Sterile filtered through a 0,2 μm filter. Lyophilized from PBS pH 7.2
Bio-activity: Recombinant human M-CSF was determined by its dose-dependent effect on the proliferation of M-NFS-60 cells. The ED50 value for this effect is typically< 2 ng/ml corresponding to specific activity>5 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.
Molecular Mass: Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted molecular mass of 20.7 kDa. The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE.
Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
Purity: Greater than 95% by SDS-PAGE gel analysis.
Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. The solution can be further diluted into aliquots in aquous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
Official Symbol: CSF1
Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1;
Gene ID: 1435
mRNA Refseq: NM_000757
Protein Refseq: NP_000748
MIM: 120420
UniProt ID: P09603
Chromosome Location: 1p21-p13
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Integrins in angiogenesis, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem;
Function: cytokine activity; growth factor activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding; protein homodimerization activity;

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