| Description : |
M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological ativity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells. |
| Source : |
Barley grain (Hordeum vulgare) |
| Species : |
Human |
| Tag : |
His |
| Form : |
Sterile filtered through a 0,2 μm filter. Lyophilized from PBS pH 7.2 |
| Bio-activity : |
Recombinant human M-CSF was determined by its dose-dependent effect on the proliferation of M-NFS-60 cells. The ED50 value for this effect is typically< 2 ng/ml corresponding to specific activity>5 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines. |
| Molecular Mass : |
Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted molecular mass of 20.7 kDa. The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE. |
| Endotoxin : |
Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg). |
| Purity : |
Greater than 95% by SDS-PAGE gel analysis. |
| Storage : |
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. The solution can be further diluted into aliquots in aquous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
