Recombinant Rat Interleukin 12b
Cat.No. : | Il12b-86R |
Product Overview : | Recombinant Rat Interleukin-12 produced inSf9insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. The IL-12 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Il12b-86R |
Description : | IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity. |
Source : | Baculovirus |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 95.8% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein (1 mg/ml) was lyophilized with no additives. |
Solubility : | It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Specific Activity : | The ED50was determined by the stimulation of IFN-gamma production by murine splenocyes co-stimulated with IL-12 and was found to be < 0.1 ng/ml, corresponding to a specific activity of >1 x 107. |
Stability : | Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL12 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | Il12b interleukin 12b [ Rattus norvegicus ] |
Synonyms : | Il12b; interleukin 12b; Il12; interleukin-12 p40 |
Gene ID : | 64546 |
mRNA Refseq : | NM_022611 |
Protein Refseq : | NP_072133 |
UniProt ID : | Q62712 |
Chromosome Location : | 10q21 |
Pathway : | Allograft rejection; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus |
Function : | cytokine activity; contributes_to cytokine activity; cytokine receptor activity; contributes_to growth factor activity; interleukin-12 alpha subunit binding; protein binding; protein homodimerization activity |
Products Types
◆ Lysates | ||
IL12B-1000MCL | Recombinant Marmoset IL12B cell lysate | +Inquiry |
IL12B-1101MCL | Recombinant Mouse IL12B cell lysate | +Inquiry |
IL12B-2731HCL | Recombinant Human IL12B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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