Recombinant Rat TF cell lysate
Cat.No. : | TF-001RCL |
Product Overview : | Rat TF / Transferrin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer. |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Tf transferrin [ Rattus norvegicus ] |
Official Symbol : | TF |
Synonyms : | TF; transferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin; liver regeneration-related protein LRRG03; Tfn; Trf; MGC93500; |
Gene ID : | 24825 |
mRNA Refseq : | NM_001013110 |
Protein Refseq : | NP_001013128 |
Pathway : | Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; |
Function : | ferric iron binding; ferric iron transmembrane transporter activity; metal ion binding; |
Products Types
◆ Native Protein | ||
TF-103H | Native Human Apotransferrin | +Inquiry |
TF-132B | Native Bovine Transferrin | +Inquiry |
TF-321H | Native Human Transferrin Rhodamine | +Inquiry |
◆ Lysates | ||
TF-1208MCL | Recombinant Mouse TF cell lysate | +Inquiry |
TF-2542HCL | Recombinant Human TF cell lysate | +Inquiry |
TF-1554SCL | Recombinant Sus scrofa (Pig) TF cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0813 | TF (Human) Chromogenic Activity Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, abnormalities in TF proteins can serve as diagnostic markers for certain diseases.
Yes, some drugs target TF proteins, especially in the treatment of cancer.
TF proteins can trigger or suppress immune responses, making them relevant in understanding autoimmune diseases.
TF proteins are vital for understanding disease mechanisms and can be targeted for therapeutic purposes.
Yes, various assays, like ELISA and Western blot, can be used to quantify TF protein levels.
Customer Reviews (3)
Write a reviewTheir team of experts is not only knowledgeable about the TF protein but is also dedicated to assisting researchers like myself in overcoming any challenges that may arise.
This high-quality protein is crucial for the success of my experiments, ensuring that I obtain reliable and meaningful scientific findings.
The TF protein is of exceptional quality, making it a perfect fit for my experimental needs.
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