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Recombinant Human IL17RB cell lysate

Cat.No. : IL17RB-549HCL
Product Overview : Human IL17RB / IL-17 Receptor B derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL17RB interleukin 17 receptor B [ Homo sapiens ]
Official Symbol : IL17RB
Synonyms : IL17RB; interleukin 17 receptor B; IL17BR, interleukin 17B receptor; interleukin-17 receptor B; CRL4; EVI27; IL17RH1; IL-17RB; IL-17Rh1; IL-17B receptor; IL-17 receptor B; cytokine receptor CRL4; IL-17 receptor homolog 1; cytokine receptor-like 4; interleukin 17B receptor; interleukin-17B receptor; interleukin 17 receptor homolog 1; IL17BR; MGC5245;
Gene ID : 55540
mRNA Refseq : NM_018725
Protein Refseq : NP_061195
MIM : 605458
UniProt ID : Q9NRM6
Chromosome Location : 3p21.1
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : cytokine receptor activity; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can you elaborate on the techniques employed to study the role of IL17RB in tissue-specific inflammation and immune cell recruitment? 11/17/2021

Animal models, flow cytometry, and tissue-specific knockout strategies uncover IL17RB's involvement in inflammation and cell recruitment.

How do researchers manipulate IL17RB expression and function in cellular and animal models to understand its contribution to autoimmune diseases? 07/10/2021

CRISPR/Cas9, siRNA, and gene editing techniques manipulate IL17RB to investigate its role in autoimmune disease pathogenesis.

Could you explain how patient samples and clinical data are utilized to investigate the correlation between IL17RB expression and disease severity in various inflammatory disorders? 01/06/2020

Biomarker analysis, patient stratification, and clinical correlation studies investigate IL17RB's association with disease severity.

What molecular and cellular assays are employed to explore the interaction between IL17RB and other immune receptors or cytokines? 09/15/2019

Co-receptor binding assays, protein-protein interaction studies, and functional rescue experiments reveal IL17RB's interaction partners.

What experimental strategies are used to dissect the involvement of IL17RB in tissue repair processes, such as wound healing and fibrosis? 05/23/2019

In vitro wound healing assays, fibroblast activation studies, and tissue regeneration models probe IL17RB's contribution to tissue repair.

What techniques are used to study the impact of IL17RB inhibition or activation on immune responses and inflammation in experimental settings? 07/29/2018

Blocking antibodies, receptor activation assays, and cytokine profiling reveal IL17RB's impact on immune responses.

How is the potential of IL17RB as a therapeutic target evaluated in preclinical studies, and what challenges exist in translating these findings into clinical applications? 12/31/2017

Animal models, therapeutic antibody studies, and safety assessments evaluate IL17RB-targeted therapies and clinical translation challenges.

How is the ligand binding affinity of IL17RB to different IL17 family members determined experimentally? 12/02/2017

Surface plasmon resonance, ELISA, and competitive binding assays determine IL17RB's affinity for various IL17 family members.

How do researchers investigate the role of IL17RB in cancer and tumor microenvironment modulation, and what experimental models are commonly used? 08/23/2017

Tumor xenograft models, immune cell infiltration assays, and gene expression analysis explore IL17RB's role in cancer biology.

What experimental methods are used to investigate the downstream signaling pathways activated by IL17RB, and how are these pathways integrated with other immune responses? 04/18/2017

Phosphorylation assays, gene expression profiling, and co-immunoprecipitation studies elucidate IL17RB-mediated signaling networks.

Customer Reviews (3)

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Reviews
10/07/2022

    High stability.

    06/23/2022

      Good for flow cytometry.

      05/20/2017

        Potent in anti-viral assays.

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