Recombinant Human IRAK4 cell lysate

• Cat.No.: IRAK4-615HCL
• Product Overview: Human IRAK4 / IRAK-4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: IRAK4 interleukin-1 receptor-associated kinase 4 [ Homo sapiens ]
• Official Symbol: IRAK4
• Synonyms: IRAK4; interleukin-1 receptor-associated kinase 4; NY REN 64; renal carcinoma antigen NY-REN-64; IPD1; REN64; IRAK-4; NY-REN-64
• Gene ID: 51135
• mRNA Refseq: NM_001114182
• Protein Refseq: NP_001107654
• MIM: 606883
• UniProt ID: Q9NWZ3
• Chromosome Location: 12q12
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem
• Function: ATP binding; interleukin-1 receptor binding; magnesium ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity

Reviews

11/16/2021

Its potency and specificity make it a valuable tool for studying specific biological pathways or mechanisms.

03/01/2020

Their technical expertise and experience in the field allow them to provide invaluable guidance and assistance.

07/16/2018

They can offer insights on experimental design, optimal usage protocols, and troubleshoot any challenges that may arise during the course of the trial.

Q&As

What conditions are associated with IRAK4 dysregulation?

Rheumatoid arthritis, lupus, inflammatory bowel diseases, and certain cancers may involve IRAK4.

Are there any ongoing clinical trials involving IRAK4 inhibitors?

Yes, several trials are investigating IRAK4 inhibitors' effectiveness and safety in various diseases.

What are the methods used to target IRAK4 in treatment?

Small molecule inhibitors and monoclonal antibodies are primary methods for modulating IRAK4 activity.

How does the expression of IRAK4 differ in healthy individuals and those with autoimmune diseases?

Individuals with autoimmune diseases often show increased IRAK4 expression compared to healthy individuals.

How does IRAK4 inhibition impact the immune response?

By blocking IRAK4, the overactive immune response in certain diseases can be suppressed.