Recombinant Human IL9 cell lysate

• Cat.No.: IL9-618HCL
• Product Overview: Human IL-9 / Interleukin-9 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: IL9 interleukin 9 [ Homo sapiens ]
• Official Symbol: IL9
• Synonyms: IL9; interleukin 9; interleukin-9; homolog of mouse T cell and mast cell growth factor 40; HP40; IL 9; P40; p40 cytokine; p40 T cell and mast cell growth factor; T cell growth factor p40; cytokine P40; T-cell growth factor p40; p40 T-cell and mast cell growth factor; IL-9
• Gene ID: 3578
• mRNA Refseq: NM_000590
• Protein Refseq: NP_000581
• MIM: 146931
• UniProt ID: P15248
• Chromosome Location: 5q31-q35
• Pathway: Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; growth factor activity; interleukin-9 receptor binding

Reviews

08/04/2022

The anti-interference ability of IL9 was excellent in Western blot, which avoided the interference problem in the experiment.

04/14/2019

This protein has high catalytic efficiency, high safety and high expression level, and is a precious resource in the field of scientific research.

02/28/2019

The reproducibility of IL9 is very good, which can reduce the error in the experiment and improve the repeatability of the experiment.

Q&As

How is the clinical trial of IL9 progressing?

Several clinical trials are currently underway to evaluate the safety and effectiveness of IL9 in treating conditions such as asthma and allergies. However, the research is still in its early stages and more data are needed.

How to obtain IL9?

IL9 can be synthesized in the laboratory by biotechnological methods. Before clinical use, it needs to go through strict quality control and testing.

What is the action mechanism of IL9?

By binding to specific receptors, IL9 can activate relevant signaling pathways and regulate the development and function of immune cells. It can affect the activity of a variety of immune cells, such as T cells and macrophages.

Howthe relationship between IL9 and other related cytokines?

IL9 has cross-action with other cytokines, which can regulate the expression and function of each other. For example, IL9 can promote the production of IL4 and IL13, and IL4 and IL13 can also promote the secretion of IL9.

What are the clinical applications of IL9?

IL9 can be used to treat certain inflammatory diseases, such as asthma and skin allergies. It can regulate the response of the immune system and reduce the symptoms of related diseases.

What is the mechanism of action of IL9 in the treatment of asthma?

The mechanism of action of IL9 in asthma treatment is not fully understood, but studies have shown that it can reduce the infiltration of inflammatory cells and the release of inflammatory mediators, thereby reducing the symptoms of asthma.