Recombinant Canine IL2RB cell lysate
Cat.No. : | IL2RB-741CCL |
Product Overview : | Canine IL2RB / IL2 Receptor beta derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Canine |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | IL2RB interleukin 2 receptor, beta [ Canis lupus familiaris ] |
Official Symbol : | IL2RB |
Synonyms : | interleukin-2 receptor subunit beta; IL 2/15 receptor beta chain |
Gene ID : | 403439 |
mRNA Refseq : | NM_001286850 |
Protein Refseq : | NP_001273779 |
Chromosome Location : | chromosome: 10 |
Pathway : | Cytokine Signaling in Immune system, organism-specific biosystem; IL-2 Signaling Pathway, organism-specific biosystem |
Products Types
◆ Recombinant Protein | ||
IL2RB-5195H | Recombinant Human IL2RB Protein | +Inquiry |
IL2RB-2310H | Recombinant Human IL2RB Protein, His-tagged | +Inquiry |
IL2RB-2069R | Recombinant Rhesus Macaque IL2RB Protein, His (Fc)-Avi-tagged | +Inquiry |
IL2RB-453C | Recombinant Canine IL2RB Protein, His-tagged | +Inquiry |
IL2RB-454R | Recombinant Rhesus IL2RB Protein, Fc-tagged | +Inquiry |
◆ Lysates | ||
IL2RB-744MCL | Recombinant Mouse IL2RB cell lysate | +Inquiry |
IL2RB-2906HCL | Recombinant Human IL2RB cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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- Q&As
- Reviews
Q&As (6)
Ask a questionIt is an artificial protein prepared by genetic engineering techniques that can mimic the natural IL-2R beta subunit and is used to explore how to treat cancer and autoimmune diseases by regulating the IL-2 signaling pathway.
There has shown good therapeutic effect in the treatment of some tumors (such as lung cancer, melanoma, etc.), but the specific efficacy varies with the patient's condition, treatment timing and other factors.
This protein can simulate the natural IL-2R beta subunit and participate in the IL-2 signaling pathway, thereby enhancing the body's immunity.
IL2RB is associated with a variety of cancers (such as gastric cancer, lung cancer, liver cancer, lymphoma, etc.) and autoimmune diseases (such as rheumatoid arthritis, systemic lupus erythematosus, etc.).
IL2RB can be used as a new therapeutic method, but it does not mean that it can replace traditional therapeutic methods. In the course of specific treatment, different treatment methods should be considered according to the condition.
IL2RB may produce some side effects, including fever, headache, nausea, vomiting, etc., but the incidence of different side effects is low.
Customer Reviews (3)
Write a reviewImmunogenicity is very weak and does not cause an immune response.
Detection method is very sensitive and can detect trace amounts of protein.
Preparation process is very simple and can be easily prepared.
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