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Recombinant Cynomolgus TNFSF4 cell lysate

Cat.No. : TNFSF4-857CCL
Product Overview : Cynomolgus OX-40L / TNFSF4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Cynomolgus
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TNFSF4 tumor necrosis factor (ligand) superfamily, member 4 [ Macaca fascicularis ]
Official Symbol : TNFSF4
Synonyms : tumor necrosis factor (ligand) superfamily, member 4; hypothetical protein
Gene ID : 102141877
mRNA Refseq : XM_005540022
Protein Refseq : XP_005540079
Chromosome Location : chromosome: 1
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How do genetic variations in TNFSF4 affect immune function? 01/09/2022

Genetic changes in TNFSF4 affect immune regulation and autoimmune disease susceptibility.

What is the primary role of TNFSF4 in immune system regulation? 08/16/2021

TNFSF4 (OX40L) regulates immune responses and T-cell activation.

How does TNFSF4 contribute to inflammatory responses? 05/11/2020

It activates T-cells, stimulating inflammatory cytokine production.

What role does TNFSF4 play in autoimmune disorders? 09/12/2019

TNFSF4 is implicated in autoimmune disease development by promoting immune cell activation.

How does TNFSF4 interact with other cytokines and receptors in the immune system? 06/09/2019

TNFSF4 interacts with OX40 and other receptors, influencing immune signaling.

What potential therapeutic applications arise from targeting TNFSF4 in immune-related diseases? 10/04/2018

Targeting TNFSF4 could treat autoimmune and inflammatory diseases by modulating the immune system.

What is the impact of altered TNFSF4 activity on chronic inflammation? 03/29/2017

Altered TNFSF4 activity contributes to chronic inflammation.

Customer Reviews (3)

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Reviews
12/28/2022

    Efficient service, accelerates research productivity.

    05/13/2018

      Outstanding accuracy, essential for research quality.

      02/12/2018

        Trusted for research integrity, a critical research asset.

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