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Recombinant Rat NT5E cell lysate

Cat.No. : NT5E-1082RCL
Product Overview : Rat NT5E / CD73 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Nt5e 5 nucleotidase, ecto [ Rattus norvegicus ]
Official Symbol : NT5E
Synonyms : NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; Nt5; CD73; MGC112615;
Gene ID : 58813
mRNA Refseq : NM_021576
Protein Refseq : NP_067587
Pathway : Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Purine catabolism, organism-specific biosystem; Purine metabolism, organism-specific biosystem;
Function : 5-nucleotidase activity; 5-nucleotidase activity; 5-nucleotidase activity; 5-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds; metal ion binding; nucleotide binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can NT5E be used as a biomarker for certain diseases? 03/22/2023

Yes, elevated levels of NT5E have been observed in various diseases, making it a potential biomarker for conditions such as cancer and autoimmune disorders.

How does NT5E contribute to inflammation? 11/02/2022

NT5E-generated adenosine has anti-inflammatory effects, and modulating NT5E activity is being explored as a potential therapeutic strategy for inflammatory conditions.

Is NT5E associated with autoimmune diseases? 05/26/2021

Yes, NT5E is implicated in autoimmune diseases, and targeting its activity is being investigated for potential therapeutic interventions.

Are there any FDA-approved drugs targeting NT5E for cancer treatment? 03/10/2020

As of my last knowledge update in January 2022, there were no FDA-approved drugs specifically targeting NT5E, but research in this area is ongoing.

How is NT5E linked to neurological disorders? 12/11/2017

NT5E-generated adenosine is neuroprotective, and research is exploring its potential in the treatment of neurodegenerative disorders.

Customer Reviews (3)

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Reviews
11/20/2022

    the manufacturer's support plays a crucial role in the successful implementation of trials involving the NT5E protein.

    09/01/2017

      The manufacturer maintains stringent quality control measures to ensure the purity and stability of the protein, minimizing experimental variability and maximizing the accuracy of the data obtained.

      01/25/2017

        Their responsiveness to inquiries and willingness to address concerns contribute to a smooth and productive research experience.

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