Recombinant Mouse FLT3LG cell lysate
Cat.No. : | FLT3LG-1387MCL |
Product Overview : | Mouse FLT3L / Flt3 ligand derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Flt3l FMS-like tyrosine kinase 3 ligand [ Mus musculus ] |
Official Symbol : | Flt3l |
Synonyms : | Ly72L; Flt3lg; fms-related tyrosine kinase 3 ligand; flt3 ligand |
Gene ID : | 14256 |
mRNA Refseq : | NM_013520 |
Protein Refseq : | NP_038548 |
UniProt ID : | P49772 |
Chromosome Location : | 7 B2-C; 7 29.14 cM |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Pathways in cancer, organism-specific biosystem |
Products Types
◆ Recombinant Protein | ||
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Flt3l-188M | Recombinant Mouse FMS-like Tyrosine Kinase 3 Ligand | +Inquiry |
Flt3l-26M | Active Recombinant Mouse Flt3l Protein (Gly27-Arg188), C-His tagged, Animal-free, Carrier-free | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionFLT3L promotes the differentiation of hematopoietic progenitor cells into various blood cell lineages, contributing to the diversity of blood cell types.
Common side effects may include flu-like symptoms, fatigue, and gastrointestinal disturbances, but the severity varies among individuals.
FLT3L is a crucial factor in the development and maturation of dendritic cells, playing a role in immune response regulation.
FLT3L supports the maintenance and self-renewal of hematopoietic stem cells in the bone marrow, influencing the overall balance of blood cell production.
Yes, elevated FLT3L levels can serve as a prognostic marker, helping to predict the likelihood of relapse and overall survival in leukemia patients.
Customer Reviews (3)
Write a reviewI am confident in the manufacturer's ability to deliver outstanding technical support.
By incorporating the FLT3L protein into my experiments, I am confident that I am utilizing a product that meets the highest standards of quality and performance.
Its high purity and integrity provide the necessary assurance for consistent and reliable results, enabling me to achieve precise and meaningful outcomes in my research.
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