Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at BIO-Europe Spring|March 18–20, 2024|Booth #34

Recombinant Rat EFNB2 cell lysate

Cat.No. : EFNB2-1540RCL
Product Overview : Rat EFNB2 / EphrinB2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Efnb2 ephrin B2 [ Rattus norvegicus ]
Official Symbol : EFNB2
Synonyms : EFNB2; ephrin B2;
Gene ID : 306636
mRNA Refseq : NM_001107328
Protein Refseq : NP_001100798
Pathway : Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem;
Function : ephrin receptor binding; ephrin receptor binding; receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
What effect does EphB6 overexpression have on EFNB2? 05/06/2022

EphB6 overexpression up-regulates the expression of its ligand EFNB2.

What diseases is EFNB2 involved in? 01/02/2022

EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

What is the biological function of EFNB2? 05/05/2021

EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

Where is EFNB2 mainly expressed? 04/10/2021

EFNB2 is widely expressed in tumor vasculature.

What is the association between EFNB2 and BUC cells? 03/12/2021

EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

How is EphB6 related to EFNB2? 07/09/2019

EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.

Customer Reviews (3)

Write a review
Reviews
01/05/2022

    During the experiment, the technician helped me solve the problems in a timely and accurate manner.

    01/02/2022

      The EFNB2 proteins purchased from your company is suitable for WB.

      01/23/2021

        Received high quality protein products and obtained satisfactory experimental results.

        Ask a Question for All EFNB2 Products

        Required fields are marked with *

        My Review for All EFNB2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends