Recombinant Rat B2M cell lysate
Cat.No. : | B2M-1550RCL |
Product Overview : | Rat B2M / Beta-2-microglobulin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | B2m beta-2 microglobulin [ Rattus norvegicus ] |
Official Symbol : | B2M |
Synonyms : | B2M; beta-2 microglobulin; beta-2-microglobulin; |
Gene ID : | 24223 |
mRNA Refseq : | NM_012512 |
Protein Refseq : | NP_036644 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; Immune System, organism-specific biosystem; |
Function : | molecular_function; |
Products Types
◆ Recombinant Protein | ||
B2M-569R | Recombinant Rat B2M Protein, His (Fc)-Avi-tagged | +Inquiry |
B2M-104H | Recombinant Human B2M Protein, His-tagged | +Inquiry |
B2M-27H | Recombinant Human B2M Protein, C-6His-Avi tagged, Biotinylated | +Inquiry |
B2M-105R | Recombinant Rat B2M Protein, His and Strep-tagged | +Inquiry |
B2M-07H | Recombinant Human B2M Protein, His-tagged(C-ter) | +Inquiry |
◆ Native Protein | ||
B2M-5366H | Native Human Beta-2-Microglobulin | +Inquiry |
B2M-13H | Native Human B2M | +Inquiry |
B2M-8046H | Native Human Beta 2 MicroGlobulin | +Inquiry |
◆ Lysates | ||
B2M-1512CCL | Recombinant Cynomolgus B2M cell lysate | +Inquiry |
B2M-1656MCL | Recombinant Mouse B2M cell lysate | +Inquiry |
B2M-2204HCL | Recombinant Human B2M cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe normal reference range for B2M varies depending on age, gender, weight, individual differences, and other factors. In general, the normal reference range is between 0~3.0mg/L.
Causes of reduced B2M include liver and kidney failure, tuberculosis, HIV infection, rheumatoid arthritis, systemic lupus erythematosus and other diseases.
B2M plays an important role in the diagnosis and prognosis assessment of malignancies such as lymphoma and multiple myeloma. Elevated B2M levels can also indicate worsening or recurrence of certain autoimmune diseases, such as systemic lupus erythematosus.
It has a variety of physiological functions in the immune system, including inducing immune response, participating in lymphocyte proliferation and differentiation, regulating immune tolerance, and participating in tumor immune surveillance.
The levels can be affected by a variety of factors, such as cancer, inflammation, autoimmune diseases, infections, medications, etc.
This is associated with many diseases, including lymphoma, normal stress hydrocephalus, HIV infection, rheumatoid arthritis, nephrotic syndrome, cirrhosis, systemic lupus erythematosus, etc.
Customer Reviews (3)
Write a reviewTo meet the needs of biological activity experiments
High purity, no impurities.
The expression level is high, which can meet the daily experimental needs.
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