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Recombinant Mouse CTSS cell lysate

Cat.No. : CTSS-1563MCL
Product Overview : Mouse CTSS / Cathepsin-S derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Ctss cathepsin S [ Mus musculus ]
Official Symbol : CTSS
Synonyms : CTSS; cathepsin S; Cat S;
Gene ID : 13040
mRNA Refseq : NM_021281
Protein Refseq : NP_067256
Pathway : Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved biosystem; Tuberculosis, organism-specific biosystem;
Function : cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How can the function of CTSS proteins be studied by gene knockout or knockdown techniques? 04/17/2022

The function of CTSS proteins can be studied through gene knockout or knockdown techniques, such as using techniques such as CRISPR-Cas9 or RNAi. These techniques can create cellular models of missing or reduced CTSS proteins to observe their effects on cell death, among other things.

How can I detect the expression level of CTSS protein? 02/05/2022

Expression level of CTSS protein can be detected by immunohistochemistry, western blotting, and real-time quantitative PCR.

How is CTSS protein involved in the process of apoptosis? 10/06/2021

CTSS proteins promote cell death by degrading proteins inside cells. During apoptosis, CTSS proteins degrade key proteins within cells, leading to cell death.

Are the interactions between CTSS proteins and other proteins regulated? 05/04/2021

The interaction between CTSS proteins and other proteins is regulated by the formation of complexes or the regulation of signal transduction pathways. These interactions can be influenced by a variety of factors, such as ion concentrations, neurotransmitters, and growth factors.

What is the relationship between the expression level of CTSS protein and the occurrence and progression of diseases? 02/08/2021

Some studies have shown that the expression level of CTSS protein may be associated with the onset and progression of certain diseases. For example, in some cancers, the expression level of the CTSS protein may change.

In which organelles is CTSS protein expressed? 09/18/2020

It is mainly expressed in lysosomes, which is one of the major proteases in lysosomes.

Customer Reviews (3)

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Reviews
11/01/2021

    High catalytic efficiency, short time, saving time.

    06/13/2021

      The solubility is very good and can be easily dissolved in various buffers.

      03/27/2021

        Anti-pollution ability is very strong, not easy to be polluted.

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