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Recombinant Human IL1RL1 cell lysate

Cat.No. : IL1RL1-1883HCL
Product Overview : Human IL1RL1 / ST2 ( isoform A ) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL1RL1 interleukin 1 receptor-like 1 [ Homo sapiens ]
Official Symbol : IL1RL1
Synonyms : IL1RL1; interleukin 1 receptor-like 1; interleukin-1 receptor-like 1; DER4; FIT 1; homolog of mouse growth stimulation expressed; IL33R; ST2; ST2L; ST2V; T1; growth stimulation-expressed; interleukin 1 receptor-related protein; homolog of mouse growth stimulation-expressed; FIT-1; MGC32623;
Gene ID : 9173
mRNA Refseq : NM_003856
Protein Refseq : NP_003847
MIM : 601203
UniProt ID : Q01638
Chromosome Location : 2q12
Function : cytokine receptor activity; interleukin-1 receptor activity; interleukin-33 binding; protein binding; receptor activity; receptor signaling protein activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Are there any splice variants of IL1RL1? 02/20/2022

IL1RL1 has multiple splice variants that differ in signaling capabilities.

What is the role of IL1RL1 in allergic responses? 11/15/2021

IL1RL1 suppresses allergic responses by inhibiting mast cell activation.

Does IL1RL1 have a role in autoimmune diseases? 10/09/2021

IL1RL1 is implicated in rheumatoid arthritis.

How does IL1RL1 affect cytokine production? 11/21/2020

IL1RL1 increases the production of IL-4 and IL-13.

What is the subcellular localization of IL1RL1? 08/13/2020

IL1RL1 is primarily membrane-bound.

Does IL1RL1 influence T-cell differentiation? 02/29/2020

IL1RL1 promotes Th2 cell differentiation.

How does IL1RL1 interact with its ligands? 01/17/2020

IL1RL1 binds to IL-33 and IL-36 to initiate signaling.

Is IL1RL1 expression altered in any cancers? 05/09/2018

IL1RL1 expression is altered in lung and breast cancer.

What post-translational modifications occur on IL1RL1? 03/28/2017

IL1RL1 undergoes glycosylation and phosphorylation.

How is IL1RL1 expression regulated in asthmatic conditions? 08/23/2016

IL1RL1 expression is upregulated in asthmatic conditions.

Customer Reviews (3)

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Reviews
07/25/2019

    High specificity in ELISA.

    01/30/2017

      Suitable for flow cytometry.

      07/13/2016

        Effective in allergy models.

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