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Recombinant Human SEMA4A cell lysate

Cat.No. : SEMA4A-1979HCL
Product Overview : Human SEMA4A / Semaphorin-4A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : SEMA4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [ Homo sapiens ]
Official Symbol : SEMA4A
Synonyms : SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2;
Gene ID : 64218
mRNA Refseq : NM_001193300
Protein Refseq : NP_001180229
MIM : 607292
UniProt ID : Q9H3S1
Chromosome Location : 1q22
Pathway : Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem;
Function : receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the impact of altered SEMA4A activity on neurodegenerative and autoimmune diseases? 07/26/2022

Altered SEMA4A activity is associated with the progression of neurodegenerative and autoimmune conditions.

What potential therapeutic applications arise from targeting SEMA4A in neurological and immune disorders? 05/10/2022

Targeting SEMA4A offers potential for treating a range of neurological and immune-mediated disorders.

What role does SEMA4A play in immune regulation? 02/25/2022

SEMA4A is involved in immune system regulation, affecting immune cell activation and responses.

What is the primary role of SEMA4A in nervous system development? 12/22/2020

SEMA4A, a semaphorin, is crucial for guiding axon development and neural connectivity in the nervous system.

How does SEMA4A contribute to axon guidance? 11/18/2019

It plays a key role in directing axonal growth and neuronal network formation.

How does SEMA4A interact with other signaling molecules? 01/29/2018

SEMA4A works with various signaling pathways, coordinating developmental and immune processes.

How do genetic variations in SEMA4A affect neural and immune functions? 10/08/2017

Genetic changes in SEMA4A can lead to impairments in neural development and immune system functioning.

Customer Reviews (3)

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10/29/2022

    Swift and accurate service, dependable for data accuracy.

    05/22/2022

      Invaluable support for insights, a research game-changer.

      10/12/2019

        Reliable partner in experiments, boosts research efficiency.

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