Recombinant Mouse F2 cell lysate
Cat.No. : | F2-2124MCL |
Product Overview : | Mouse FII / F2 / Thrombin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | F2 coagulation factor II [ Mus musculus ] |
Official Symbol : | F2 |
Synonyms : | F2; coagulation factor II; prothrombin; Cf2; FII; Cf-2; thrombin; |
Gene ID : | 14061 |
mRNA Refseq : | NM_010168 |
Protein Refseq : | NP_034298 |
Pathway : | Blood Clotting Cascade, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Common Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; |
Function : | calcium ion binding; catalytic activity; hydrolase activity; peptidase activity; protein binding; receptor binding; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type peptidase activity; thrombospondin receptor activity; |
Products Types
◆ Recombinant Protein | ||
F2-3610H | Recombinant Human F2 Protein, GST-tagged | +Inquiry |
F2-955H | Recombinant Human F2 Protein, MYC/DDK-tagged | +Inquiry |
F2-882H | Recombinant Human F2 Protein, His (Fc)-Avi-tagged | +Inquiry |
F2-789H | Recombinant Human F2 Protein | +Inquiry |
F2-366R | Recombinant Rat F2 Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
Prothrombin-271B | Active Native Bovine Prothrombin Frag 1 | +Inquiry |
F2-303R | Native Rat Thrombin | +Inquiry |
F2-5286R | Native Rat Coagulation Factor II | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionThe F2 gene plays a crucial role in the synthesis of vitamin K-dependent clotting factors, including prothrombin. Disruptions in this process, often due to genetic or nutritional factors, can contribute to coagulation disorders, impacting hemostasis and blood clotting.
Transcriptional regulation of the F2 gene involves factors like vitamin K-dependent gamma-carboxylation. Hepatocytes synthesize prothrombin under the influence of vitamin K and other coagulation factors.
The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.
Genetic variations in F2, including polymorphisms, can influence prothrombin function. Certain variants may predispose individuals to thrombotic or bleeding disorders.
Genetic variations in the F2 gene can influence prothrombin levels, affecting an individual's propensity for thrombotic events. Certain variants may predispose individuals to a higher or lower risk of abnormal blood clot formation.
Customer Reviews (3)
Write a reviewQuality control is evident. This product ensures accuracy in our studies.
Our lab swears by this protein. Fast shipping is a definite bonus.
Consistently reliable. The shipping speed is appreciated in our fast-paced lab.
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