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Recombinant Human IL1RAPL1 cell lysate

Cat.No. : IL1RAPL1-2784HCL
Product Overview : Human IL-1R8 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL1RAPL1 interleukin 1 receptor accessory protein-like 1 [ Homo sapiens ]
Official Symbol : IL1RAPL1
Synonyms : IL1RAPL1; interleukin 1 receptor accessory protein-like 1; IL1RAPL, mental retardation, X linked 10 , mental retardation, X linked 21 , mental retardation, X linked 34 , MRX10, MRX21, MRX34; interleukin-1 receptor accessory protein-like 1; IL1R8; OPHN4; TIGIRR 2; IL1RAPL-1; IL-1RAPL-1; IL-1-RAPL-1; oligophrenin-4; interleukin 1 receptor-8; mental retardation, X-linked 10; X-linked interleukin-1 receptor accessory protein-like 1; three immunoglobulin domain-containing IL-1 receptor-related 2; MRX10; MRX21; MRX34; IL1RAPL; TIGIRR-2;
Gene ID : 11141
mRNA Refseq : NM_014271
Protein Refseq : NP_055086
MIM : 300206
UniProt ID : Q9NZN1
Chromosome Location : Xp22.1-p21.3
Function : NOT interleukin-1 binding; protein binding; receptor activity; transmembrane signaling receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can IL1RAPL1 mutations be linked to specific neurological disorders? 06/27/2022

Linked to intellectual disabilities.

How does IL1RAPL1 expression vary in different brain regions? 01/27/2022

Higher in the hippocampus and cortex.

How does IL1RAPL1 expression change during neural development? 10/01/2021

Increases during early development, decreases in adulthood.

How does IL1RAPL1 interact with other members of the IL-1 receptor family? 07/27/2021

Forms heterodimers with IL-1RI.

How does IL1RAPL1 interact with PSD-95 in neurons? 12/22/2020

Binds directly to the PDZ domains of PSD-95.

What is the role of IL1RAPL1 in synaptic plasticity? 09/28/2020

Involved in excitatory synapse formation.

Is IL1RAPL1 expression affected by neuroinflammatory conditions? 02/26/2020

Upregulated in neuroinflammatory conditions.

Does IL1RAPL1 have any alternative splicing variants? 04/09/2019

Yes, multiple isoforms exist.

What post-translational modifications occur on IL1RAPL1? 08/01/2018

Subject to ubiquitination.

Does IL1RAPL1 have a role in dendritic spine morphology? 06/12/2018

Affects spine density and shape.

Customer Reviews (3)

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Reviews
07/08/2022

    Effective in inflammation studies.

    03/20/2020

      High specificity.

      07/30/2017

        Suitable for cytokine assays.

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