Recombinant Human IL1RAPL1 cell lysate
Cat.No. : | IL1RAPL1-2784HCL |
Product Overview : | Human IL-1R8 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | IL1RAPL1 interleukin 1 receptor accessory protein-like 1 [ Homo sapiens ] |
Official Symbol : | IL1RAPL1 |
Synonyms : | IL1RAPL1; interleukin 1 receptor accessory protein-like 1; IL1RAPL, mental retardation, X linked 10 , mental retardation, X linked 21 , mental retardation, X linked 34 , MRX10, MRX21, MRX34; interleukin-1 receptor accessory protein-like 1; IL1R8; OPHN4; TIGIRR 2; IL1RAPL-1; IL-1RAPL-1; IL-1-RAPL-1; oligophrenin-4; interleukin 1 receptor-8; mental retardation, X-linked 10; X-linked interleukin-1 receptor accessory protein-like 1; three immunoglobulin domain-containing IL-1 receptor-related 2; MRX10; MRX21; MRX34; IL1RAPL; TIGIRR-2; |
Gene ID : | 11141 |
mRNA Refseq : | NM_014271 |
Protein Refseq : | NP_055086 |
MIM : | 300206 |
UniProt ID : | Q9NZN1 |
Chromosome Location : | Xp22.1-p21.3 |
Function : | NOT interleukin-1 binding; protein binding; receptor activity; transmembrane signaling receptor activity; |
Products Types
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Il1rapl1-6962M | Active Recombinant Mouse Il1rapl1 protein(Met1-Thr357), hFc-tagged | +Inquiry |
IL1RAPL1-776H | Recombinant Human IL1RAPL1, Fc tagged | +Inquiry |
IL1RAPL1-1575H | Recombinant Human IL1RAPL1 protein, His & T7-tagged | +Inquiry |
◆ Lysates | ||
IL1RAPL1-2740HCL | Recombinant Human IL1RAPL1 cell lysate | +Inquiry |
IL1RAPL1-1688MCL | Recombinant Mouse IL1RAPL1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionLinked to intellectual disabilities.
Higher in the hippocampus and cortex.
Increases during early development, decreases in adulthood.
Forms heterodimers with IL-1RI.
Binds directly to the PDZ domains of PSD-95.
Involved in excitatory synapse formation.
Upregulated in neuroinflammatory conditions.
Yes, multiple isoforms exist.
Subject to ubiquitination.
Affects spine density and shape.
Customer Reviews (3)
Write a reviewEffective in inflammation studies.
High specificity.
Suitable for cytokine assays.
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