Recombinant Mouse CTSD cell lysate
Cat.No. : | CTSD-3023MCL |
Product Overview : | Mouse Cathepsin D / CTSD derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Ctsd cathepsin D [ Mus musculus ] |
Official Symbol : | CTSD |
Synonyms : | CTSD; cathepsin D; CD; CatD; |
Gene ID : | 13033 |
mRNA Refseq : | NM_009983 |
Protein Refseq : | NP_034113 |
Pathway : | Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem; |
Function : | aspartic-type endopeptidase activity; endopeptidase activity; hydrolase activity; peptidase activity; peptide binding; |
Products Types
◆ Recombinant Protein | ||
CTSD-877H | Recombinant Human CTSD Protein, His-tagged | +Inquiry |
Ctsd-2369M | Recombinant Mouse Ctsd Protein, His-tagged | +Inquiry |
CTSD-2752H | Recombinant Human CTSD Protein, His (Fc)-Avi-tagged | +Inquiry |
CTSD-786H | Recombinant Human CTSD Protein, His-tagged | +Inquiry |
CTSD-913R | Recombinant Rhesus Macaque CTSD Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
CTSD-27858TH | Native Human CTSD | +Inquiry |
CTSD-26411TH | Active Native Human Cathepsin D protein | +Inquiry |
CTSD-189H | Active Native Human Cathepsin D | +Inquiry |
◆ Lysates | ||
CTSD-1735HCL | Recombinant Human CTSD cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0264 | Cathepsin D Inhibitor Screening Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThere is an association between CTSD proteins and cognitive function. In some neurodegenerative diseases, abnormal accumulation and deposition of CTSD proteins may affect neuronal signaling and synaptic function, leading to a decline in cognitive function.
In some neurodegenerative diseases, levels of the CTSD protein may change. For example, people with Alzheimer's disease may have elevated levels of the CTSD protein. These changes may help in the diagnosis of the disease and in the assessment of the course of the disease.
For people with cognitive decline, their CTSD protein levels can be assessed by a blood or cerebrospinal fluid sample. This evaluation can help doctors understand whether the cause of cognitive decline is related to a neurodegenerative disease.
It is currently no treatment that directly targets the CTSD protein. However, some medications can reduce symptoms of neurodegenerative diseases, such as cholinesterase inhibitors such as donepezil, which can increase acetylcholine levels in synapses, which can improve cognitive function.
People with Parkinson's disease may have reduced levels of the CTSD protein. This decrease may be associated with neuronal death and impaired synaptic function. In addition, CTSD protein may also be associated with motor symptoms in patients with Parkinson's disease.
CTSD proteins play a role in Alzheimer's disease, Parkinson's disease, and other neurodegenerative diseases. It may change in these diseases, leading to structural alterations and impaired signaling of neuronal synapses.
Customer Reviews (3)
Write a reviewGood at western-blot experiments.
In experiments, the biocompatibility performed well.
The experimental conditions are broad, and the experimental results are accurate and repeatable.
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