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Recombinant Human VCP 293 Cell Lysate

Cat.No.: VCP-419HCL
Description: Antigen standard for valosin-containing protein (VCP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: VCP valosin containing protein [ Homo sapiens ]
Official Symbol: VCP
Synonyms: VCP; valosin containing protein; transitional endoplasmic reticulum ATPase; IBMPFD; p97; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit; TERA; ALS14; MGC8560; MGC131997; MGC148092;
Gene ID: 7415
mRNA Refseq: NM_007126
Protein Refseq: NP_009057
MIM: 601023
UniProt ID: P55072
Chromosome Location: 9p13
Pathway: DNA Repair, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; HRD1/SEL1 ERAD complex, organism-specific biosystem; HRD1/SEL1 ERAD complex, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Legionellosis, organism-specific biosystem; Legionellosis, conserved biosystem;

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