"VASP" Related Products

Recombinant Human VASP 293 Cell Lysate

Cat.No.: VASP-426HCL
Description: Antigen standard for vasodilator-stimulated phosphoprotein (VASP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: VASP vasodilator-stimulated phosphoprotein [ Homo sapiens ]
Official Symbol: VASP
Synonyms: VASP; vasodilator-stimulated phosphoprotein;
Gene ID: 7408
mRNA Refseq: NM_003370
Protein Refseq: NP_003361
MIM: 601703
UniProt ID: P50552
Chromosome Location: 19q13.32
Pathway: Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-extracellular matrix interactions, organism-specific biosystem; Developmental Biology, organism-specific biosystem; E-cadherin signaling in keratinocytes, organism-specific biosystem;

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