Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human THBS1 293 Cell Lysate

Cat.No. : THBS1-1101HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for thrombospondin 1 (THBS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : THBS1 thrombospondin 1 [ Homo sapiens ]
Official Symbol : THBS1
Synonyms : THBS1; thrombospondin 1; thrombospondin-1; THBS; THBS 1; thrombospondin 1p180; TSP; TSP 1; TSP1; thrombospondin-1p180; TSP-1; THBS-1;
Gene ID : 7057
mRNA Refseq : NM_003246
Protein Refseq : NP_003237
MIM : 188060
UniProt ID : P07996
Chromosome Location : 15q15
Pathway : Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
In which tissues or cell types is THBS1 predominantly expressed? 09/01/2022

THBS1 is widely expressed but is upregulated in response to injury or in certain disease states.

How is THBS1 implicated in cancer progression? 07/10/2021

It can either promote or inhibit cancer progression, depending on the context.

How does THBS1 contribute to cell-matrix interactions? 04/28/2021

It binds to extracellular matrix components, influencing cell attachment and signaling.

What role does THBS1 play in angiogenesis? 09/14/2020

THBS1 acts as an angiogenesis inhibitor, hindering new blood vessel formation.

What therapeutic strategies are being explored targeting THBS1? 03/08/2020

Therapeutic strategies include targeting THBS1 to modulate its effects in cancer and wound healing.

What is the primary function of THBS1 in cellular processes? 06/17/2018

THBS1 modulates cell adhesion, proliferation, and migration.

Are there known genetic variations in THBS1 associated with specific diseases? 02/13/2018

Genetic variations in THBS1 have been linked to cardiovascular diseases and cancer risk.

Customer Reviews (3)

Write a review
Reviews
05/14/2022

    Quality exceeded my expectations.

    09/08/2021

      Satisfied customer, will return.

      11/21/2020

        Very pure, excellent results.

        Ask a Question for All THBS1 Products

        Required fields are marked with *

        My Review for All THBS1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends