Recombinant Human SNAI2 293 Cell Lysate

• Cat.No.: SNAI2-1642HCL

Specification

• Description: Antigen standard for snail homolog 2 (Drosophila) (SNAI2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SNAI2 snail homolog 2 (Drosophila) [ Homo sapiens ]
• Official Symbol: SNAI2
• Synonyms: SNAI2; snail homolog 2 (Drosophila); SLUG, slug homolog, zinc finger protein (chicken); zinc finger protein SNAI2; SLUGH1; SNAIL2; protein snail homolog 2; neural crest transcription factor SLUG; slug (chicken homolog), zinc finger protein; SLUG; WS2D; MGC10182
• Gene ID: 6591
• mRNA Refseq: NM_003068
• Protein Refseq: NP_003059
• MIM: 602150
• UniProt ID: O43623
• Chromosome Location: 8q11.21
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Direct p53 effectors, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Signaling events mediated by Stem cell factor receptor (c-Kit), organism-specific biosystem
• Function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; chromatin binding; metal ion binding; sequence-specific DNA binding; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; zinc ion binding