Recombinant Human RALBP1 293 Cell Lysate

• Cat.No.: RALBP1-2542HCL

Specification

• Description: Antigen standard for ralA binding protein 1 (RALBP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RALBP1 ralA binding protein 1 [ Homo sapiens ]
• Official Symbol: RALBP1
• Synonyms: RALBP1; ralA binding protein 1; ralA-binding protein 1; RIP; RIP1; RLIP76; DNP-SG ATPase; ral-interacting protein 1; 76 kDa Ral-interacting protein; dinitrophenyl S-glutathione ATPase; RLIP1
• Gene ID: 10928
• mRNA Refseq: NM_006788
• Protein Refseq: NP_006779
• MIM: 605801
• UniProt ID: Q15311
• Chromosome Location: 18p11.22
• Pathway: EGFR1 Signaling Pathway, organism-specific biosystem; Pancreatic cancer, organism-specific biosystem; Pancreatic cancer, conserved biosystem; Pathways in cancer, organism-specific biosystem; Regulation of CDC42 activity, organism-specific biosystem; Regulation of RAC1 activity, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem
• Function: ATPase activity; ATPase activity, coupled to movement of substances; GTPase activator activity; Rac GTPase activator activity; Rac GTPase binding; Ral GTPase binding; protein binding