"PKM" Related Products


Recombinant Human PKM2 293 Cell Lysate

Cat.No.: PKM2-3153HCL
Description: Antigen standard for pyruvate kinase, muscle (PKM2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PKM2 pyruvate kinase, muscle [ Homo sapiens ]
Official Symbol: PKM2
Synonyms: PKM2; pyruvate kinase, muscle; pyruvate kinase isozymes M1/M2; OIP3; PK3; THBP1; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic; PKM; TCB; CTHBP; MGC3932;
Gene ID: 5315
mRNA Refseq: NM_002654
Protein Refseq: NP_002645
MIM: 179050
UniProt ID: P14618
Chromosome Location: 15q23
Pathway: Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem; Glycolysis (Embden-Meyerhof pathway), glucose =>pyruvate, organism-specific biosystem; Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem;
Function: ATP binding; magnesium ion binding; nucleotide binding; potassium ion binding; protein binding; pyruvate kinase activity; transferase activity;

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