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Recombinant Mouse Pkm Protein, His-tagged

Cat.No.: Pkm-968M
Product Overview: Recombinant mouse PKM (NP_001240812.1) (Pro2-Pro531) was expressed with a polyhistidine tag at the N-terminus.
Source: Baculovirus-Insect cells
Species: Mouse
Tag: His
Predicted N Terminal: His
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant mouse PKM consists of 548 amino acids and predicts a molecular mass of 60 KDa. It migrates as an approximately 59 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Pkm pyruvate kinase, muscle [ Mus musculus ]
Official Symbol: Pkm
Synonyms: Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
Gene ID: 18746
mRNA Refseq: NM_001253883
Protein Refseq: NP_001240812
MIM:
UniProt ID: P52480
Chromosome Location: 9 B; 9 32.03 cM
Pathway: Adenine ribonucleotide biosynthesis, IMP =>ADP,ATP, organism-specific biosystem; Biosynthesis of amino acids, organism-specific biosystem; Carbon metabolism, conserved biosystem
Function: ADP binding; metal ion binding; poly(A) RNA binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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