| Cat. No. : |
PTK2-38H |
| Description : |
FAK is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. |
| Source : |
Baculovirus, Sf9 insect cells. |
| Amino Acid Sequence : |
MAPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRHNQTSL YKKAGTMAAA YLDPNLNHTP NSSTKTHLGT GMERSPGAME RVLKVFHYFE SNSEPTTWAS IIRHGDATDVRGIIQKIVDS HKVKHVACYG FRLSHLRSEE VHWLHVDMGV SSVREKYELA HPPEEWKYEL RIRYLPKGFL NQFTEDKPTL NFFYQQVKSD YMLEIADQVD QEIALKLGCL EIRRSYWEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS VKAKTLRKLI QQTFRQFANL NREESILKFF EILSPVYRFD KECFKCALGS SWIISVELAI GPEEGISYLT DKGCNPTHLA DFTQVQTIQY SNSEDKDRKG MLQLKIAGAP EPLTVTAPSL TIAENMADLI DGYCRLVNGT SQSFIIRPQK EGERALPSIP KLANSEKQGM RTHAVSVSET DDYAEIIDEE DTYTMPSTRD YEIQRERIEL GRCIGEGQFG DVHQGIYMSP ENPALAVAIK TCKNCTSDSV REKFLQEALT MRQFDHPHIV KLIGVITENP VWIIMELCTL GELRSFLQVR KYSLDLASLI LYAYQLSTAL AYLESKRFVH RDIAARNVLV SSNDCVKLGD FGLSRYMEDS TYYKASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL MTKCWAYDPS RRPRFTELKA QLSTILEEEK AQQEERMRME SRRQATVSWD SGGSDEAPPK PSRPGYPSPR SSEGFYPSPQ HMVQTNHYQV SGYPGSHGIT AMAGSIYPGQ ASLLDQTDSW NHRPQEIAMW QPNVEDSTVL DLRGIGQVLP THLMEERLIR QQQEMEEDQR WLEKEERFLK PDVRLSRGSI DREDGSLQGP IGNQHIYQPVGKPDPAAPPK KPPRPGAPGH LGSLASLSSP ADSYNEGVKL QPQEISPPPT ANLDRSNDKV YENVTGLVKA VIEMSSKIQP APPEEYVPMV KEVGLALRTLLATVDETIPL LPASTHREIE MAQKLLNSDL GELINKMKLA QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL LDVIDQARLK MLGQTRPH. |
| Dilution Buffer : |
20mM Tris pH-7.5, 10% Glycerol, 0.02% Triton X-100, 0.1mg/ml BSA, 0.5mM Na3VO4, 2mM DTT. |
| Purity : |
Greater than 70% as determined by SDS-PAGE. |
| Formulation : |
FADK is supplied in 50mM Tris pH-7.5, 150mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, & 50% Glycerol. |
| Biological Activity : |
72 nmole of phosphate transferred to poly [Glu,Tyr] 4:1 substrate/minute/mg of total protein at 30°C. Activity determined at a final protein concentration of 4μg/ml. |
| Storage : |
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C to -80°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
