Recombinant Human PDPK1 293 Cell Lysate

• Cat.No.: PDPK1-3321HCL

Specification

• Description: Antigen standard for 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PDPK1 3-phosphoinositide dependent protein kinase-1 [ Homo sapiens ]
• Official Symbol: PDPK1
• Synonyms: PDPK1; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; PDK1; PkB kinase; PkB-like 1; PkB kinase like gene 1; PRO0461; MGC20087; MGC35290
• Gene ID: 5170
• mRNA Refseq: NM_002613
• Protein Refseq: NP_002604
• MIM: 605213
• UniProt ID: O15530
• Chromosome Location: 16p13.3
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Activation of PKB, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem
• Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding; kinase activity; nucleotide binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity