"PDE7B" Related Products

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Recombinant Human PDE7B 293 Cell Lysate

Cat.No.: PDE7B-3340HCL
Description: Antigen standard for phosphodiesterase 7B (PDE7B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PDE7B phosphodiesterase 7B [ Homo sapiens ]
Official Symbol: PDE7B
Synonyms: PDE7B; phosphodiesterase 7B; cAMP-specific 3,5-cyclic phosphodiesterase 7B; rolipram-insensitive phosphodiesterase type 7; high-affinity cAMP-specific 3,5-cyclic phosphodiesterase; bA472E5.1; MGC88256;
Gene ID: 27115
mRNA Refseq: NM_018945
Protein Refseq: NP_061818
MIM: 604645
UniProt ID: Q9NP56
Chromosome Location: 6q23-q24
Pathway: G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem;
Function: 3,5-cyclic-AMP phosphodiesterase activity; 3,5-cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity;

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