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Recombinant Human PAX6 293 Cell Lysate

Cat.No.: PAX6-3415HCL
Description: Antigen standard for paired box 6 (PAX6), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PAX6 paired box 6 [ Homo sapiens ]
Official Symbol: PAX6
Synonyms: PAX6; paired box 6; AN2, paired box gene 6 (aniridia, keratitis); paired box protein Pax-6; AN; aniridia; keratitis; D11S812E; WAGR; oculorhombin; aniridia type II protein; paired box homeotic gene-6; AN2; MGDA; MGC17209;
Gene ID: 5080
mRNA Refseq: NM_000280
Protein Refseq: NP_000271
MIM: 607108
UniProt ID: P26367
Chromosome Location: 11p13
Pathway: CDC42 signaling events, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Maturity onset diabetes of the young, organism-specific biosystem; Maturity onset diabetes of the young, conserved biosystem; Metabolism, organism-specific biosystem;
Function: DNA binding; R-SMAD binding; RNA polymerase II core promoter sequence-specific DNA binding; histone acetyltransferase binding; protein binding; protein kinase binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; ubiquitin-protein ligase activity;

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