Recombinant Human NONO 293 Cell Lysate
Cat.No. : | NONO-3766HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for non-POU domain containing, octamer-binding (NONO), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | NONO non-POU domain containing, octamer-binding [ Homo sapiens ] |
Official Symbol : | NONO |
Synonyms : | NONO; non-POU domain containing, octamer-binding; non POU domain containing, octamer binding; non-POU domain-containing octamer-binding protein; NMT55; non Pou domain containing octamer (ATGCAAAT) binding protein; NRB54; Nuclear RNA binding protein; 54 kD; P54; P54NRB; p54(nrb); 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex, 52 kDa subunit; non-POU domain-containing octamer (ATGCAAAT) binding protein; |
Gene ID : | 4841 |
mRNA Refseq : | NM_001145408 |
Protein Refseq : | NP_001138880 |
MIM : | 300084 |
UniProt ID : | Q15233 |
Chromosome Location : | Xq13.1 |
Pathway : | Circadian rhythm pathway, organism-specific biosystem; mRNA processing, organism-specific biosystem; |
Function : | DNA binding; RNA binding; identical protein binding; nucleotide binding; protein binding; |
Products Types
◆ Recombinant Protein | ||
NONO-2496H | Recombinant Human NONO Protein, His-tagged | +Inquiry |
NONO-2497H | Recombinant Human NONO Protein, His tagged, Avi-Biotinylated | +Inquiry |
NONO-2885R | Recombinant Rhesus Macaque NONO Protein, His (Fc)-Avi-tagged | +Inquiry |
NONO-2496HB | Recombinant Human NONO Protein, His tagged, Avi-Biotinylated | +Inquiry |
NONO-3679R | Recombinant Rat NONO Protein, His (Fc)-Avi-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (6)
Ask a questionThis protein is widely expressed in a variety of tissues and organs, including the brain, heart, liver, kidneys, and muscles.
Yes, NONO protein is involved in the regulation of RNA splicing and plays an important role in the splicing process by interacting with other proteins.
NONO protein is expressed in the liver and is involved in the regulation of signaling pathways related to liver function. However, further research is needed to determine its specific role.
NONO protein interacts with DNA repair-related proteins and is involved in the repair process of DNA double-strand breaks.
The protein has been implicated in some neurological diseases, such as motor neuron disease, but its specific role and mechanism still need to be further studied.
NONO protein has been implicated in a variety of diseases, including leukemia, neurological diseases such as motor neuron disease, and progeria.
Customer Reviews (3)
Write a reviewThe stability of NONO has good adaptability under different temperature and pH conditions.
NONO has important application value in the field of drug research and development, and has achieved certain research results.
Experiments with NONO are easy to use and do not require complex procedures.
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