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Recombinant Human NONO 293 Cell Lysate

Cat.No. : NONO-3766HCL
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  • Gene Information
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Description : Antigen standard for non-POU domain containing, octamer-binding (NONO), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : NONO non-POU domain containing, octamer-binding [ Homo sapiens ]
Official Symbol : NONO
Synonyms : NONO; non-POU domain containing, octamer-binding; non POU domain containing, octamer binding; non-POU domain-containing octamer-binding protein; NMT55; non Pou domain containing octamer (ATGCAAAT) binding protein; NRB54; Nuclear RNA binding protein; 54 kD; P54; P54NRB; p54(nrb); 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex, 52 kDa subunit; non-POU domain-containing octamer (ATGCAAAT) binding protein;
Gene ID : 4841
mRNA Refseq : NM_001145408
Protein Refseq : NP_001138880
MIM : 300084
UniProt ID : Q15233
Chromosome Location : Xq13.1
Pathway : Circadian rhythm pathway, organism-specific biosystem; mRNA processing, organism-specific biosystem;
Function : DNA binding; RNA binding; identical protein binding; nucleotide binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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In which tissues or organs is NONO protein highly expressed? 06/15/2022

This protein is widely expressed in a variety of tissues and organs, including the brain, heart, liver, kidneys, and muscles.

Is NONO protein involved in RNA splicing regulation? 03/24/2022

Yes, NONO protein is involved in the regulation of RNA splicing and plays an important role in the splicing process by interacting with other proteins.

Is NONO protein involved in liver function? 02/08/2022

NONO protein is expressed in the liver and is involved in the regulation of signaling pathways related to liver function. However, further research is needed to determine its specific role.

Is there a relationship between NONO protein and DNA damage repair? 01/10/2022

NONO protein interacts with DNA repair-related proteins and is involved in the repair process of DNA double-strand breaks.

What is the role of NONO protein in neurological diseases? 11/30/2021

The protein has been implicated in some neurological diseases, such as motor neuron disease, but its specific role and mechanism still need to be further studied.

What diseases are NONO associated with? 01/21/2021

NONO protein has been implicated in a variety of diseases, including leukemia, neurological diseases such as motor neuron disease, and progeria.

Customer Reviews (3)

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Reviews
08/08/2023

    The stability of NONO has good adaptability under different temperature and pH conditions.

    04/01/2022

      NONO has important application value in the field of drug research and development, and has achieved certain research results.

      04/01/2020

        Experiments with NONO are easy to use and do not require complex procedures.

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