Recombinant Human NEDD4 293 Cell Lysate

• Cat.No.: NEDD4-3886HCL

Specification

• Description: Antigen standard for neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NEDD4 neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: NEDD4
• Synonyms: NEDD4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down regulated 4; E3 ubiquitin-protein ligase NEDD4; KIAA0093; MGC176705; NEDD4 1; receptor potentiating factor 1; RPF1; NEDD-4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4; NEDD4-1
• Gene ID: 4734
• mRNA Refseq: NM_006154
• Protein Refseq: NP_006145
• MIM: 602278
• UniProt ID: P46934
• Chromosome Location: 15q
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem
• Function: RNA polymerase binding; acid-amino acid ligase activity; beta-2 adrenergic receptor binding; ligase activity; phosphoserine binding; phosphothreonine binding; proline-rich region binding; proline-rich region binding; protein binding; protein domain specific binding; sodium channel inhibitor activity; ubiquitin binding; ubiquitin-protein ligase activity