"NDEL1" Related Products

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Recombinant Human NDEL1 293 Cell Lysate

Cat.No.: NDEL1-3936HCL
Description: Antigen standard for nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NDEL1 nudE nuclear distribution E homolog (A. nidulans)-like 1 [ Homo sapiens ]
Official Symbol: NDEL1
Synonyms: NDEL1; nudE nuclear distribution E homolog (A. nidulans)-like 1; nudE nuclear distribution gene E homolog (A. nidulans) like 1; nuclear distribution protein nudE-like 1; MITAP1; NUDEL; protein Nudel; endooligopeptidase A; mitosin-associated protein 1; mitosin-associated protein MITAP1; nudE nuclear distribution gene E homolog-like 1; EOPA; DKFZp451M0318;
Gene ID: 81565
mRNA Refseq: NM_001025579
Protein Refseq: NP_001020750
MIM: 607538
UniProt ID: Q9GZM8
Chromosome Location: 17p13.1
Pathway: Aurora A signaling, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; Lissencephaly gene (LIS1) in neuronal migration and development, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem;
Function: alpha-tubulin binding; beta-tubulin binding; microtubule binding; oligopeptidase activity; protein binding;

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