Recombinant Human MRE11A 293 Cell Lysate

• Cat.No.: MRE11A-4211HCL

Specification

• Description: Antigen standard for MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: MRE11A
• Synonyms: MRE11A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); meiotic recombination (S. cerevisiae) 11 homolog A , MRE11; double-strand break repair protein MRE11A; AT like disease; ATLD; AT-like disease; MRE11 homolog 1; MRE11 homolog A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; DNA recombination and repair protein; HNGS1; MRE11; MRE11B
• Gene ID: 4361
• mRNA Refseq: NM_005590
• Protein Refseq: NP_005581
• MIM: 600814
• UniProt ID: P49959
• Chromosome Location: 11q21
• Pathway: Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; DNA Repair, organism-specific biosystem; DNA damage response, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Homologous Recombination Repair, organism-specific biosystem
• Function: 3-5 exonuclease activity; contributes_to ATP-dependent DNA helicase activity; contributes_to DNA binding; double-stranded DNA binding; endodeoxyribonuclease activity; hydrolase activity; manganese ion binding; nuclease activity; protein C-terminus binding; protein binding; single-stranded DNA specific endodeoxyribonuclease activity