Recombinant Human LIPA 293 Cell Lysate
Cat.No. : | LIPA-4727HCL |
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Description : | Antigen standard for lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | LIPA lipase A, lysosomal acid, cholesterol esterase [ Homo sapiens ] |
Official Symbol : | LIPA |
Synonyms : | LIPA; lipase A, lysosomal acid, cholesterol esterase; lysosomal acid lipase/cholesteryl ester hydrolase; CESD; LAL; Wolman disease; sterol esterase; cholesteryl esterase; lysosomal acid lipase; cholesterol ester hydrolase; acid cholesteryl ester hydrolase; |
Gene ID : | 3988 |
mRNA Refseq : | NM_000235 |
Protein Refseq : | NP_000226 |
MIM : | 613497 |
UniProt ID : | P38571 |
Chromosome Location : | 10q23.2-q23.3 |
Pathway : | Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Steroid biosynthesis, organism-specific biosystem; Steroid biosynthesis, conserved biosystem; |
Function : | hydrolase activity; lipase activity; lipase activity; sterol esterase activity; sterol esterase activity; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionLIPA protein deficiency impairs the breakdown of cholesterol esters and triglycerides, leading to their accumulation in various tissues and organs.
Diagnosis of LIPA protein deficiency is typically made through genetic testing and measurement of LIPA enzyme activity in blood samples.
Patients with LIPA protein deficiency are often advised to follow a low-fat, high-carbohydrate diet to help manage their lipid levels.
LIPA protein deficiency can result in the accumulation of cholesterol esters and triglycerides in the liver, leading to liver damage and dysfunction.
Yes, researchers are actively studying LIPA protein deficiency and potential therapeutic approaches, including gene therapy and novel enzyme replacement strategies.
Customer Reviews (3)
Write a reviewIts reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various fields of study.
The reliable performance of the LIPA protein in ELISA and its compatibility with protein electron microscopy structure analysis make it an excellent choice for a wide range of research studies.
Their knowledgeable and responsive team assists in troubleshooting and finding solutions to any experimental challenges that may arise.
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