Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human HMGB2 293 Cell Lysate

Cat.No. : HMGB2-5477HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for high-mobility group box 2 (HMGB2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : HMGB2 high mobility group box 2 [ Homo sapiens ]
Official Symbol : HMGB2
Synonyms : HMGB2; high mobility group box 2; high mobility group (nonhistone chromosomal) protein 2 , high mobility group box 2 , HMG2; high mobility group protein B2; HMG-2; high-mobility group box 2; high mobility group protein 2; high-mobility group (nonhistone chromosomal) protein 2; HMG2;
Gene ID : 3148
mRNA Refseq : NM_001130688
Protein Refseq : NP_001124160
MIM : 163906
UniProt ID : P26583
Chromosome Location : 4q31
Pathway : Activation of DNA fragmentation factor, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem;
Function : DNA binding; DNA binding, bending; RAGE receptor binding; chemoattractant activity; damaged DNA binding; double-stranded DNA binding; protein binding; protein domain specific binding; sequence-specific DNA binding transcription factor activity; single-stranded DNA binding; transcription regulatory region DNA binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
Is there a treatment strategy for HMGB2, and what are there? 06/24/2022

Therapeutic strategies for HMGB2 include inhibition of its activity, modulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against HMGB2 to inhibit its aberrant activity in disease, or the regulation of HMGB2 expression through gene knockout or overexpression techniques.

What diseases are HMGB2 associated with? 07/06/2021

Studies have shown that the aberrant expression of HMGB2 is associated with the occurrence of a variety of diseases, including cancer, autoimmune diseases, and neurodegenerative diseases. In cancer, high expression of HMGB2 is closely related to tumor initiation, development, and metastasis.

Which biomolecules can be used as molecular markers of HMGB2? 11/05/2020

Certain biomolecules can serve as molecular markers of HMGB2, such as mRNA or protein of HMGB2 detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

Which drugs can act on HMGB2? 09/21/2020

At present, some drugs have been developed to act on HMGB2, including some small molecule inhibitors and antibody drugs. These drugs can inhibit the activity of HMGB2 or modulate its expression, thereby treating diseases associated with abnormal expression of HMGB2.

How is the role of HMGB2 assessed in disease? 07/13/2020

The role of HMGB2 in disease can be assessed through in-depth analysis of the patient's genomic data, combined with the results of studies in cell and animal models. This can help doctors better understand the patient's condition and develop more effective treatment plans.

What are the detection methods for HMGB2? 06/12/2020

Detection methods for HMGB2 include immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect the expression level of HMGB2 in tissues or cells to assess its status in the disease.

Customer Reviews (3)

Write a review
Reviews
04/02/2023

    After using HMGB2, my experiments are very reproducible, which is convenient for subsequent data analysis and experimental operation.

    09/01/2022

      The structure is stable and not easy to mutate.

      08/24/2020

        HMGB2 has a very high catalytic activity, which can greatly reduce the time and energy required for chemical reactions, and its economic value is immeasurable.

        Ask a Question for All HMGB2 Products

        Required fields are marked with *

        My Review for All HMGB2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends