Recombinant Human HMGB2 293 Cell Lysate
Cat.No. : | HMGB2-5477HCL |
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- Gene Information
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Description : | Antigen standard for high-mobility group box 2 (HMGB2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | HMGB2 high mobility group box 2 [ Homo sapiens ] |
Official Symbol : | HMGB2 |
Synonyms : | HMGB2; high mobility group box 2; high mobility group (nonhistone chromosomal) protein 2 , high mobility group box 2 , HMG2; high mobility group protein B2; HMG-2; high-mobility group box 2; high mobility group protein 2; high-mobility group (nonhistone chromosomal) protein 2; HMG2; |
Gene ID : | 3148 |
mRNA Refseq : | NM_001130688 |
Protein Refseq : | NP_001124160 |
MIM : | 163906 |
UniProt ID : | P26583 |
Chromosome Location : | 4q31 |
Pathway : | Activation of DNA fragmentation factor, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; |
Function : | DNA binding; DNA binding, bending; RAGE receptor binding; chemoattractant activity; damaged DNA binding; double-stranded DNA binding; protein binding; protein domain specific binding; sequence-specific DNA binding transcription factor activity; single-stranded DNA binding; transcription regulatory region DNA binding; |
Products Types
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Hmgb2-1626R | Recombinant Rat Hmgb2 Protein, His-tagged | +Inquiry |
Hmgb2-3412M | Recombinant Mouse Hmgb2 Protein, Myc/DDK-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionTherapeutic strategies for HMGB2 include inhibition of its activity, modulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against HMGB2 to inhibit its aberrant activity in disease, or the regulation of HMGB2 expression through gene knockout or overexpression techniques.
Studies have shown that the aberrant expression of HMGB2 is associated with the occurrence of a variety of diseases, including cancer, autoimmune diseases, and neurodegenerative diseases. In cancer, high expression of HMGB2 is closely related to tumor initiation, development, and metastasis.
Certain biomolecules can serve as molecular markers of HMGB2, such as mRNA or protein of HMGB2 detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.
At present, some drugs have been developed to act on HMGB2, including some small molecule inhibitors and antibody drugs. These drugs can inhibit the activity of HMGB2 or modulate its expression, thereby treating diseases associated with abnormal expression of HMGB2.
The role of HMGB2 in disease can be assessed through in-depth analysis of the patient's genomic data, combined with the results of studies in cell and animal models. This can help doctors better understand the patient's condition and develop more effective treatment plans.
Detection methods for HMGB2 include immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect the expression level of HMGB2 in tissues or cells to assess its status in the disease.
Customer Reviews (3)
Write a reviewAfter using HMGB2, my experiments are very reproducible, which is convenient for subsequent data analysis and experimental operation.
The structure is stable and not easy to mutate.
HMGB2 has a very high catalytic activity, which can greatly reduce the time and energy required for chemical reactions, and its economic value is immeasurable.
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