Recombinant Human HMGA1 293 Cell Lysate

• Cat.No.: HMGA1-5480HCL

Specification

• Description: Antigen standard for high mobility group AT-hook 1 (HMGA1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: HMGA1 high mobility group AT-hook 1 [ Homo sapiens ]
• Official Symbol: HMGA1
• Synonyms: HMGA1; high mobility group AT-hook 1; high mobility group (nonhistone chromosomal) protein isoforms I and Y , HMGIY; high mobility group protein HMG-I/HMG-Y; HMG-I(Y); high mobility group protein R; high mobility group protein A1; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; HMG-R; HMGIY; HMGA1A; MGC4242; MGC4854; MGC12816
• Gene ID: 3159
• mRNA Refseq: NM_002131
• Protein Refseq: NP_002122
• MIM: 600701
• UniProt ID: P17096
• Chromosome Location: 6p21
• Pathway: 2-LTR circle formation, organism-specific biosystem; APOBEC3G mediated resistance to HIV-1 infection, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Autointegration results in viral DNA circles, organism-specific biosystem; Disease, organism-specific biosystem; Early Phase of HIV Life Cycle, organism-specific biosystem; HIV Infection, organism-specific biosystem
• Function: 5-deoxyribose-5-phosphate lyase activity; AT DNA binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; peroxisome proliferator activated receptor binding; protein binding; retinoic acid receptor binding; retinoid X receptor binding; sequence-specific DNA binding transcription factor activity; transcription factor binding