"GFAP" Related Products

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Recombinant Human GFAP 293 Cell Lysate

Cat.No.: GFAP-5955HCL
Description: Antigen standard for glial fibrillary acidic protein (GFAP), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GFAP glial fibrillary acidic protein [ Homo sapiens ]
Official Symbol: GFAP
Synonyms: GFAP; glial fibrillary acidic protein; FLJ45472; intermediate filament protein; FLJ42474;
Gene ID: 2670
mRNA Refseq: NM_001131019
Protein Refseq: NP_001124491
MIM: 137780
UniProt ID: P14136
Chromosome Location: 17q21
Pathway: Nuclear signaling by ERBB4, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by ERBB4, organism-specific biosystem;
Function: integrin binding; structural constituent of cytoskeleton;

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