"FZR1" Related Products

Recombinant Human FZR1 293 Cell Lysate

Cat.No.: FZR1-6087HCL
Description: Antigen standard for fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FZR1 fizzy/cell division cycle 20 related 1 (Drosophila) [ Homo sapiens ]
Official Symbol: FZR1
Synonyms: FZR1; fizzy/cell division cycle 20 related 1 (Drosophila); fizzy-related protein homolog; CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1; KIAA1242; CDC20-like 1b; cdh1/Hct1 homolog; CDC20-like protein 1;
Gene ID: 51343
mRNA Refseq: NM_001136197
Protein Refseq: NP_001129669
MIM: 603619
UniProt ID: Q9UM11
Chromosome Location: 19p13.3
Pathway: APC/C complex, organism-specific biosystem; APC/C complex, conserved biosystem; APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem;
Function: kinase activity; protein binding;

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