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Recombinant Human FYN 293 Cell Lysate

Cat.No.: FYN-6091HCL
Description: Antigen standard for FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FYN FYN oncogene related to SRC, FGR, YES [ Homo sapiens ]
Official Symbol: FYN
Synonyms: FYN; FYN oncogene related to SRC, FGR, YES; tyrosine-protein kinase Fyn; MGC45350; SLK; SYN; src-like kinase; proto-oncogene Syn; c-syn protooncogene; proto-oncogene c-Fyn; src/yes-related novel; tyrosine kinase p59fyn(T); OKT3-induced calcium influx regulator; p59-FYN;
Gene ID: 2534
mRNA Refseq: NM_153048
Protein Refseq: NP_694593
MIM: 137025
UniProt ID: P06241
Chromosome Location: 6q21
Pathway: Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha-synuclein signaling, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem;
Function: ATP binding; ephrin receptor binding; glycoprotein binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; protein tyrosine kinase activity;

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