Recombinant Human FTH1 293 Cell Lysate
Cat.No. : | FTH1-6128HCL |
- Specification
- Gene Information
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Description : | Antigen standard for ferritin, heavy polypeptide 1 (FTH1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | FTH1 ferritin, heavy polypeptide 1 [ Homo sapiens ] |
Official Symbol : | FTH1 |
Synonyms : | FTH1; ferritin, heavy polypeptide 1; FTHL6; ferritin heavy chain; apoferritin; FHC; FTH; PIG15; placenta immunoregulatory factor; PLIF; proliferation inducing protein 15; ferritin H subunit; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein; MGC104426; |
Gene ID : | 2495 |
mRNA Refseq : | NM_002032 |
Protein Refseq : | NP_002023 |
UniProt ID : | P02794 |
Chromosome Location : | 11q13 |
Pathway : | Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; Porphyrin and chlorophyll metabolism, organism-specific biosystem; |
Function : | ferric iron binding; ferroxidase activity; iron ion binding; oxidoreductase activity; protein binding; |
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◆ Native Protein | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, FTH1 levels in blood or tissues are sometimes used as diagnostic markers for iron-related disorders, helping to assess iron status in patients.
FTH1's role in iron metabolism is relevant in cancer, where altered iron levels can influence tumor growth. Researchers explore FTH1 as a potential target for cancer therapy.
FTH1 is associated with inflammation, as its expression can be influenced by inflammatory signals. This link is of interest in understanding diseases with both iron dysregulation and inflammation.
FTH1 expression levels are being explored as potential prognostic markers in diseases like cancer, providing insights into disease progression and outcomes.
Customer Reviews (3)
Write a reviewAs a researcher, utilizing FTH1 protein in trials offers numerous advantages that contribute to the successful completion of experiments and the acquisition of valuable data.
manufacturers offer customization options, such as alternative conjugation methods or tailored formulations, allowing researchers to optimize FTH1 protein to their specific experimental needs.
This ensures the consistency and reproducibility of experimental results, promoting confidence in the obtained data.
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