"FOXQ1" Related Products

Recombinant Human FOXQ1 293 Cell Lysate

Cat.No.: FOXQ1-6144HCL
Description: Antigen standard for forkhead box Q1 (FOXQ1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FOXQ1 forkhead box Q1 [ Homo sapiens ]
Official Symbol: FOXQ1
Synonyms: FOXQ1; forkhead box Q1; forkhead box protein Q1; HFH1; HFH-1; HNF-3/forkhead-like protein 1; winged helix/forkhead transcription factor; hepatocyte nuclear factor 3 forkhead homolog 1;
Gene ID: 94234
mRNA Refseq: NM_033260
Protein Refseq: NP_150285
MIM: 612788
UniProt ID: Q9C009
Chromosome Location: 6p25
Function: DNA binding, bending; cysteine-type endopeptidase regulator activity involved in apoptotic process; double-stranded DNA binding; estrogen receptor binding; sequence-specific DNA binding; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding; ubiquitin conjugating enzyme binding;

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