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Recombinant Human ERMAP 293 Cell Lysate

Cat.No. : ERMAP-6549HCL
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Description : Antigen standard for erythroblast membrane-associated protein (Scianna blood group) (ERMAP), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ERMAP erythroblast membrane-associated protein (Scianna blood group) [ Homo sapiens ]
Official Symbol : ERMAP
Synonyms : ERMAP; erythroblast membrane-associated protein (Scianna blood group); erythroblast membrane associated protein , erythroblast membrane associated protein (RD and SC blood groups) , Radin blood group , RD, SC, Scianna blood group; erythroid membrane-associated protein; Radin blood group (Rd); Scianna blood group (Sc); radin blood group antigen; scianna blood group antigen; RD; SC; PRO2801; MGC118810; MGC118811; MGC118812; MGC118813;
Gene ID : 114625
mRNA Refseq : NM_001017922
Protein Refseq : NP_001017922
MIM : 609017
UniProt ID : Q96PL5
Chromosome Location : 1p34

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Are there any ongoing clinical trials related to ERMAP protein and anemia? 10/09/2021

Clinical trials investigating the role of ERMAP protein in anemia and potential therapeutic interventions are currently underway, aiming to advance our understanding and develop new treatment options.

What are the diagnostic implications of ERMAP mutations in anemia? 08/03/2021

ERMAP mutations can be identified through genetic testing, which can aid in the diagnosis and classification of anemia, as well as guide treatment strategies.

How does ERMAP protein contribute to the regulation of iron metabolism? 04/06/2018

ERMAP protein may play a role in the regulation of iron uptake and utilization by red blood cells, which is critical for the maintenance of normal hemoglobin levels and prevention of iron-deficiency anemia.

How is ERMAP protein involved in the etiology of anemia? 01/11/2017

ERMAP protein is essential for the proper development and maturation of red blood cells. Mutations in the ERMAP gene can lead to various types of anemia, such as hereditary spherocytosis.

How does ERMAP protein contribute to the pathophysiology of hemolytic anemia? 04/24/2016

ERMAP protein is involved in maintaining the structural integrity of red blood cells, and mutations in the ERMAP gene can lead to hemolytic anemia due to increased fragility and destruction of red blood cells.

Customer Reviews (3)

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Reviews
11/27/2020

    Their prompt and effective responses help ensure smooth and efficient progress, indeed solving any problem that arises.

    10/02/2016

      The knowledgeable and experienced support team is readily available to address any questions or concerns that researchers may have throughout their experimental journey.

      07/02/2016

        Its stability and reliability warrant its inclusion in my research protocols, leading to meaningful and conclusive results.

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