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Recombinant Human EHMT1 293 Cell Lysate

Cat.No.: EHMT1-6685HCL
Description: Antigen standard for euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EHMT1 euchromatic histone-lysine N-methyltransferase 1 [ Homo sapiens ]
Official Symbol: EHMT1
Synonyms: EHMT1; euchromatic histone-lysine N-methyltransferase 1; euchromatic histone methyltransferase 1; histone-lysine N-methyltransferase EHMT1; bA188C12.1; Eu HMTase1; FLJ12879; KIAA1876; KMT1D; H3-K9-HMTase 5; G9a like protein; G9a-like protein 1; lysine N-methyltransferase 1D; histone H3-K9 methyltransferase 5; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; GLP; GLP1; FP13812; EUHMTASE1; Eu-HMTase1; RP11-188C12.1; DKFZp667M072;
Gene ID: 79813
mRNA Refseq: NM_001145527
Protein Refseq: NP_001138999
MIM: 607001
UniProt ID: Q9H9B1
Chromosome Location: 9
Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem;
Function: histone methyltransferase activity (H3-K27 specific); histone methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding;

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