"CYP7B1" Related Products

Recombinant Human CYP7B1 293 Cell Lysate

Cat.No.: CYP7B1-7098HCL
Description: Antigen standard for cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CYP7B1 cytochrome P450, family 7, subfamily B, polypeptide 1 [ Homo sapiens ]
Official Symbol: CYP7B1
Synonyms: CYP7B1; cytochrome P450, family 7, subfamily B, polypeptide 1; cytochrome P450, subfamily VIIB (oxysterol 7 alpha hydroxylase), polypeptide 1 , spastic paraplegia 5A (autosomal recessive) , SPG5A; 25-hydroxycholesterol 7-alpha-hydroxylase; cytochrome P450 7B1; oxysterol 7alpha-hydroxylase; oxysterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; CP7B; CBAS3; SPG5A;
Gene ID: 9420
mRNA Refseq: NM_004820
Protein Refseq: NP_004811
MIM: 603711
UniProt ID: O75881
Chromosome Location: 8q21.3
Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Endogenous sterols, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem;
Function: 25-hydroxycholesterol 7alpha-hydroxylase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxysterol 7-alpha-hydroxylase activity;

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