Recombinant Human CYP46A1 293 Cell Lysate
Cat.No. : | CYP46A1-7105HCL |
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- Gene Information
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Description : | Antigen standard for cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | CYP46A1 cytochrome P450, family 46, subfamily A, polypeptide 1 [ Homo sapiens ] |
Official Symbol : | CYP46A1 |
Synonyms : | CYP46A1; cytochrome P450, family 46, subfamily A, polypeptide 1; CYP46, cytochrome P450, subfamily 46 (cholesterol 24 hydroxylase); cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase); CP46; CYP46; |
Gene ID : | 10858 |
mRNA Refseq : | NM_006668 |
Protein Refseq : | NP_006659 |
MIM : | 604087 |
UniProt ID : | Q9Y6A2 |
Chromosome Location : | 14q32.1 |
Pathway : | Bile acid and bile salt metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Endogenous sterols, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem; |
Function : | cholesterol 24-hydroxylase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid hydroxylase activity; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionResearch suggests that altered CYP46A1 activity and expression may serve as a biomarker for neurodegenerative diseases and could aid in early detection and monitoring of disease progression.
Dysregulation of CYP46A1-mediated cholesterol metabolism has been implicated in the pathogenesis of Huntington's disease, making it a potential therapeutic target.
Research is investigating the potential of targeting CYP46A1 for therapeutic interventions, such as the development of small molecule modulators to regulate its activity.
CYP46A1 activity influences the clearance of cholesterol from the brain, which may interact with the effects of cholesterol-lowering drugs, potentially affecting their efficacy.
Some genetic variations in the CYP46A1 gene have been linked to altered CYP46A1 activity and an increased risk of neurodegenerative diseases.
Customer Reviews (3)
Write a reviewThey can also address any questions or concerns regarding the CYP46A1 protein, thereby facilitating smooth and efficient research execution.
manufacturers may offer customization options, allowing researchers to tailor the CYP46A1 protein to their specific needs.
This may include options like different formulations, protein modifications, or tagging techniques. Customization can enhance the versatility of the protein and optimize its utility in the intended research.
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