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Recombinant Human CYP46A1 293 Cell Lysate

Cat.No. : CYP46A1-7105HCL
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  • Gene Information
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Description : Antigen standard for cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CYP46A1 cytochrome P450, family 46, subfamily A, polypeptide 1 [ Homo sapiens ]
Official Symbol : CYP46A1
Synonyms : CYP46A1; cytochrome P450, family 46, subfamily A, polypeptide 1; CYP46, cytochrome P450, subfamily 46 (cholesterol 24 hydroxylase); cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase); CP46; CYP46;
Gene ID : 10858
mRNA Refseq : NM_006668
Protein Refseq : NP_006659
MIM : 604087
UniProt ID : Q9Y6A2
Chromosome Location : 14q32.1
Pathway : Bile acid and bile salt metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Endogenous sterols, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem;
Function : cholesterol 24-hydroxylase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid hydroxylase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can CYP46A1 protein be used as a biomarker for neurodegenerative diseases? 04/18/2023

Research suggests that altered CYP46A1 activity and expression may serve as a biomarker for neurodegenerative diseases and could aid in early detection and monitoring of disease progression.

How does CYP46A1 protein influence the development and progression of Huntington's disease? 02/03/2023

Dysregulation of CYP46A1-mediated cholesterol metabolism has been implicated in the pathogenesis of Huntington's disease, making it a potential therapeutic target.

Can CYP46A1 protein be targeted for therapeutic interventions in neurodegenerative diseases? 05/04/2021

Research is investigating the potential of targeting CYP46A1 for therapeutic interventions, such as the development of small molecule modulators to regulate its activity.

How does CYP46A1 protein impact the efficacy of cholesterol-lowering drugs? 11/19/2020

CYP46A1 activity influences the clearance of cholesterol from the brain, which may interact with the effects of cholesterol-lowering drugs, potentially affecting their efficacy.

Are there any genetic variations in the CYP46A1 gene associated with clinical conditions? 09/17/2018

Some genetic variations in the CYP46A1 gene have been linked to altered CYP46A1 activity and an increased risk of neurodegenerative diseases.

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11/24/2022

    They can also address any questions or concerns regarding the CYP46A1 protein, thereby facilitating smooth and efficient research execution.

    02/21/2017

      manufacturers may offer customization options, allowing researchers to tailor the CYP46A1 protein to their specific needs.

      05/08/2016

        This may include options like different formulations, protein modifications, or tagging techniques. Customization can enhance the versatility of the protein and optimize its utility in the intended research.

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