Recombinant Human CNOT4 293 Cell Lysate
Cat.No. : | CNOT4-7400HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | CNOT4 CCR4-NOT transcription complex, subunit 4 [ Homo sapiens ] |
Official Symbol : | CNOT4 |
Synonyms : | CNOT4; CCR4-NOT transcription complex, subunit 4; NOT4; CCR4-NOT transcription complex subunit 4; CLONE243; NOT4H; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; potential transcriptional repressor NOT4Hp; NOT4 (negative regulator of transcription 4, yeast) homolog; |
Gene ID : | 4850 |
mRNA Refseq : | NM_001008225 |
Protein Refseq : | NP_001008226 |
MIM : | 604911 |
UniProt ID : | O95628 |
Chromosome Location : | 7q22-qter |
Pathway : | Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Gene Expression, organism-specific biosystem; RNA degradation, organism-specific biosystem; RNA degradation, conserved biosystem; |
Function : | RNA binding; ligase activity; metal ion binding; nucleotide binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
Cnot4-2221M | Recombinant Mouse Cnot4 Protein, Myc/DDK-tagged | +Inquiry |
CNOT4-1582H | Recombinant Human CNOT4 Protein, GST-tagged | +Inquiry |
CNOT4-2039C | Recombinant Chicken CNOT4 | +Inquiry |
◆ Lysates | ||
CNOT4-7401HCL | Recombinant Human CNOT4 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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