"ATP6AP1" Related Products

Recombinant Human ATP6AP1 293 Cell Lysate

Cat.No.: ATP6AP1-8592HCL
Description: Antigen standard for ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ATP6AP1 ATPase, H+ transporting, lysosomal accessory protein 1 [ Homo sapiens ]
Official Symbol: ATP6AP1
Synonyms: ATP6AP1; ATPase, H+ transporting, lysosomal accessory protein 1; ATP6IP1, ATP6S1, ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1; V-type proton ATPase subunit S1; 16A; Ac45; CF2; ORF; VATPS1; XAP 3; XAP3; protein XAP-3; H-ATPase subunit; V-ATPase subunit S1; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; vacuolar proton pump subunit S1; ATPase, H+ transporting, lysosomal interacting protein 1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1; XAP-3; ATP6S1; ATP6IP1; MGC129781;
Gene ID: 537
mRNA Refseq: NM_001183
Protein Refseq: NP_001174
MIM: 300197
UniProt ID: Q15904
Chromosome Location: Xq28
Pathway: Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Metabolic pathways, organism-specific biosystem; Oxidative phosphorylation, organism-specific biosystem; Oxidative phosphorylation, organism-specific biosystem;
Function: ATP binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; nucleotide binding; proton-transporting ATPase activity, rotational mechanism; transporter activity;

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