Recombinant Human ASCL1 293 Cell Lysate

• Cat.No.: ASCL1-8654HCL

Specification

• Description: Antigen standard for achaete-scute complex homolog 1 (Drosophila) (ASCL1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASCL1 achaete-scute complex homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: ASCL1
• Synonyms: ASCL1; achaete-scute complex homolog 1 (Drosophila); achaete scute complex (Drosophila) homolog like 1 , achaete scute complex like 1 (Drosophila); achaete-scute homolog 1; ASH1; bHLHa46; HASH1; ASH-1; achaete scute protein; achaete-scute complex-like 1; class A basic helix-loop-helix protein 46; MASH1
• Gene ID: 429
• mRNA Refseq: NM_004316
• Protein Refseq: NP_004307
• MIM: 100790
• UniProt ID: P50553
• Chromosome Location: 12q22-q23
• Pathway: Delta-Notch Signaling Pathway, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem
• Function: DNA binding; E-box binding; bHLH transcription factor binding; double-stranded DNA binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription factor binding transcription factor activity