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Recombinant Human APLNR 293 Cell Lysate

Cat.No. : APLNR-8791HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for apelin receptor (APLNR), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : APLNR apelin receptor [ Homo sapiens ]
Official Symbol : APLNR
Synonyms : APLNR; apelin receptor; AGTRL1, angiotensin II receptor like 1; APJ; APJ (apelin) receptor; APJR; FLJ90771; APJ receptor; HG11 orphan receptor; angiotensin receptor-like 1; G protein-coupled receptor APJ; G-protein coupled receptor APJ; angiotensin II receptor-like 1; G-protein coupled receptor HG11; HG11; AGTRL1; FLJ96609; MGC45246;
Gene ID : 187
mRNA Refseq : NM_005161
Protein Refseq : NP_005152
MIM : 600052
UniProt ID : P35414
Chromosome Location : 11q12.1
Pathway : Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
Function : G-protein coupled receptor activity; receptor activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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When is the best time to treat APLNR ? 12/15/2022

At present, there is no specific optimal treatment time, which needs to be determined according to the specific situation and condition of the patient.

Is the efficacy of APLNR long-lasting? 12/12/2022

At present, there are no clear results of long-lasting efficacy studies, and more long-term clinical observations and studies are needed.

What are the potential risks and side effects of the use of recombinant APLNR? 11/26/2022

As with all drugs, there are risks and side effects associated with the use of APLNR s, such as allergic reactions and bleeding.

Can APLNR be used to prevent disease? 05/29/2022

There is no clear way to prevent use, and more research and exploration is needed.

Does APLNR affect blood lipid levels? 05/13/2022

According to studies, APLNR can raise HDL cholesterol levels and lower LDL cholesterol levels, thus affecting lipid levels.

Is APLNR recombinant immunogenic? 04/09/2022

Studies have shown that the recombinant APLNR protein is not immunogenic in most people.

Is APLNR effective in the treatment of children? 10/25/2021

At present, there are no relevant studies to prove whether APLNR is effective in the treatment of children, and more research and exploration are needed.

Can APLNR increase the therapeutic effect through its own repair mechanism? 06/02/2021

According to research, APLNR may increase the therapeutic effect by regulating its own repair mechanism.

Is there any difference in the therapeutic effect of APLNR ? 08/09/2020

Treatment effectiveness may vary and needs to be evaluated on a patient basis.

How to prepare APLNR ? 06/07/2020

Recombinant APLNR proteins can be expressed in microorganisms such as Escherichia coli using genetic engineering technology, and then extracted and purified by protein purification technology.

Has APLNR been used in clinical trials? 04/29/2020

Yes, there are currently clinical trials underway to evaluate the safety and efficacy of APLNR.

Which diseases do recombinant APLNR proteins have potential therapeutic effects? 04/26/2020

Current studies have shown that APLNR has a potential role in the treatment of diseases such as heart disease, hepatitis, tumors and kidney disease.

Is APLNR effective in the treatment of the elderly? 04/19/2020

According to research, recombinant APLNR may be more effective in the treatment of the elderly.

What is the pharmacological principle of APLNR therapy? 12/29/2019

The therapeutic effect and targeting mechanism of APLNR is mainly through binding to its receptor APJ and activating related signaling pathways.

How to determine the dose of APLNR in clinical treatment? 12/29/2019

There is no specific standard dose, and the dosage is usually determined based on patient conditions and clinical trial results.

Does APLNR have potential for long-term application? 12/06/2019

At present, there are no specific studies to show whether the long-term use of APLNR is safe and effective, and long-term clinical observation is needed.

Does APLNR have the potential to be used in combination with drugs? 09/27/2019

According to research, the combination of APLNR and other drugs may produce a more significant effect.

Is the price of APLNR expensive? 06/19/2019

Based on current research and clinical trials, APLNR s are more expensive, and more research and production are needed to reduce costs.

Can APLNR be used as an alternative to traditional drug therapy? 05/21/2019

At present, there is no detailed evidence that APLNR can completely replace traditional drug therapy, and more research and exploration are needed.

Can APLNR be administered orally? 01/27/2019

There is currently no specific method of oral administration, but relevant studies may be explored.

Customer Reviews (4)

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Reviews
11/24/2020

    APLNR is stable under various experimental conditions and is ideal for a variety of application scenarios.

    10/22/2020

      Green production process of APLNR has the characteristics of high efficiency and environmental protection, and has set a new benchmark for the industry.

      04/19/2019

        The quantitative accuracy of Western blot is very high, which can accurately determine the content of target protein, indicating that it has high accuracy and low error rate.

        03/21/2019

          Stability of APLNR is very good, and it can be carried out for long periods of experimental operation without worrying about changes and effects of its performance.

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