Recombinant Human ALDOB 293 Cell Lysate

• Cat.No.: ALDOB-8911HCL

Specification

• Description: Antigen standard for aldolase B, fructose-bisphosphate (ALDOB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALDOB aldolase B, fructose-bisphosphate [ Homo sapiens ]
• Official Symbol: ALDOB
• Synonyms: ALDOB; aldolase B, fructose-bisphosphate; fructose-bisphosphate aldolase B; aldolase 2; liver-type aldolase; aldolase B, fructose-bisphosphatase; ALDB; ALDO2
• Gene ID: 229
• mRNA Refseq: NM_000035
• Protein Refseq: NP_000026
• MIM: 612724
• UniProt ID: P05062
• Chromosome Location: 9q21.3-q22.2
• Pathway: FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Fructose catabolism, organism-specific biosystem; Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>fructose-6P, organism-specific biosystem
• Function: ATPase binding; cytoskeletal protein binding; fructose binding; fructose-bisphosphate aldolase activity; fructose-bisphosphate aldolase activity; identical protein binding; lyase activity; protein binding